Fluorescent Antibody Method of Detecting Gonorrhea in Asymptomatic Females

Fluorescent antibody test for the serological diagnosis of gonorrhea.

An indirect fluorescent antibody technique has been developed for the serological diagnosis of gonorrhea. The selected strain(s) of Neisseria gonorrhoeae possesses a heat-labile surface antigen (L-antigen). Sera are diluted 1:10, and an aliquot is hear inactivated at 59 C for 30 min. The treated and untreated aliquots are then examined for human immunoglobulin G anti-L-antigen. In a prelimiary ...

Fluorescent-antibody method useful for detecting viable but nonculturable Salmonella spp. in chlorinated wastewater.

An indirect fluorescent-antibody (IFA) technique, which employed adsorbed Behring polyvalent I O antiserum, was used to detect Salmonella spp. in environmental water systems. The IFA method used in this study detected 95% of Salmonella serotypes encountered in human infections in France, with a sensitivity threshold of 7.5 x 10(3) bacteria per ml of wastewater. Specificity was assessed by testi...

Visualization of Horse Sickness virus by the Fluorescent antibody technique

Diagnosis of Intestinal Amoebiasis with Indirect Fluorescent Antibody Test

SUMMARY The results of direct stool examination in 102 watery and bloody diarrhea patients were compared with the presence of antibodies against Entamoeba histolytica with Indirect Fluorescent Antibody Test. Besides 22 healthy individuals included in this study as control group. It was found that 22 patients (21.5%) with a sera titre higher than 1/320 bad only 9 (8.8%) positive E. Histolytica ...

Immunofluorescent Staining: the Fluorescent Antibody Method.

I. Principles of Tmmunofluorescent Staining.................................................. A. Fluorescent Antibody Labeling........................................................ B. Immunofluorescent Staining Procedures............................................... C. Controls.............................................................................. D. Nonspecific Staining................